Critical
—cm/h
Chromatography · Lab Tools
Watch the math
work.
Six focused, browser‑based tools for chromatography scientists — column & slurry design, open‑bed pressure‑flow prediction, purification protocol building, buffer recipe calculation, column qualification with HETP and asymmetry analysis, and Protein A process economics with live mass-balance and resin-cost simulation. Drag the curve on the right: the fit re‑solves live.
No sign‑up·no install
100% in‑browser — your process data never leaves your device.
Pressure – Flow
Drag the points. The Nelder–Mead solver re-fits as you move.
Packing
—cm/h
Running
—cm/h
Six calculators. One workflow.
Each tool is a focused instrument — open one, enter your numbers, read the result.
Column & Slurry
A real‑time constraint‑solving engine for routine chromatography maths — column sizing, slurry preparation, residence time, and volumetric‑to‑linear flow conversion.
- Volume, area & height
- Slurry & packing factor
- Residence time
- Flow conversion
Model
V = A·H | t = V/Q | QL = Qv·60/A
Open Bed
Enter your open‑bed pressure‑flow measurements and the Nelder‑Mead solver curve‑fits both the flow model and the bed‑compression model to predict critical, packing, and running parameters.
- Exponential flow‑rise fit
- Double‑exp compression
- Nelder‑Mead optimiser
- Critical / Packing / Running
Model
y = fa·(1 − fbx) / (L·cp)
Chromatography Protocol and Materials
A purification‑protocol builder with a bidirectional column‑parameter solver, step‑time calculation, and a chemical‑component database that auto‑fills molecular weight for buffer preparation.
- Column ID, height & volume
- Protocol steps with CV
- Chemical database (90+)
- Auto MW → weight
Model
V = CV·Vcol | W = C·MW·V
Buffer Recipe
Design a buffer to a target pH with the Henderson–Hasselbalch equation. Pick a buffer system and a preparation method — weigh both forms, titrate with NaOH/HCl, or mix stock solutions — and get a step‑by‑step recipe.
- 27 preset systems + custom
- pH, concentration & volume
- 4 preparation methods
- Temperature & ionic strength
Model
pH = pKa + log([A−]/[HA])
HETP & Asymmetry
Column qualification using the USP <621> half-height method. Computes theoretical plates, HETP, reduced plate height, and asymmetry factor with PASS / MARGINAL / FAIL verdicts — plus van Deemter curve fitting.
- Theoretical plates (N)
- Reduced plate height (h)
- Asymmetry factor (As)
- van Deemter curve fitting
Model
N = 5.54 × (VR/Wh)2 | h = HETP/dp
Protein A Process Economics
Compare three downstream Protein A capture scenarios side-by-side with live mass-balance and resin cost simulation against a 76-column hardware database spanning five column families.
- Resin cost / campaign
- Protein cost ($/g)
- Multi-scenario comparison
- Column auto-selection
Model
Cost/g = (Presin × Vbed × (n+1)) / mass