Chromatography · Lab Tools

Watch the math
work.

Six free, browser-based calculators for chromatography scientists — from column design and buffer prep to HETP qualification and Protein A economics. Drag the curve: the fit re-solves live.

Explore the calculators Start with Column & Slurry
  • No sign-up
  • No install
  • 100% in-browser — your data never leaves your device
  • Formulas cited: USP <621> · Stickel & Fotopoulos 2001

Pressure – Flow

Live fit

Drag the points. The Nelder–Mead solver re-fits as you move.

Critical
—cm/h
Packing
—cm/h
Running
—cm/h

Built for lab precision

Why AmChromaLab

6
calculators, one workflow
106
chemical compounds in the database
76columns· 24families
pre-packed & lab column database
USP <621>
+ Stickel & Fotopoulos (2001) — methods, cited
0
data uploaded — every calculation runs in your browser

How it works

  1. Open a tool in your browser
  2. Enter your numbers
  3. Read results — export a PDF report

Anonymous usage stats only — never the values you enter. Privacy Policy

Six calculators. One workflow.

Each tool is a focused instrument — open one, enter your numbers, read the result.

  1. 01 DesignColumn & Slurry
  2. 02 PredictOpen Bed
  3. 03 PlanProtocol Builder
  4. 04 PrepareBuffer Recipe
  5. 05 QualifyHETP & Asymmetry
  6. 06 CostProtein A Economics
Start here

Column & Slurry

Real-time column sizing, slurry prep, residence time, and flow conversion in one constraint-solving engine.

  • Volume, area & height
  • Slurry & packing factor
  • Residence time
  • Flow conversion
Model V = A·H  |  t = V/Q  |  QL = Qv·60/A
PDF report
Open calculator

Open Bed

Curve-fit your pressure–flow measurements to predict critical, packing, and running bed velocities.

  • Exponential flow‑rise fit
  • Double‑exp compression
  • Nelder‑Mead optimiser
  • Critical / Packing / Running
Model y = fa·(1 − fbx) / (L·cp)
PDF report
Open calculator

Protocol Builder

Build purification protocols with a bidirectional column solver and the built-in chemical database.

  • Column ID, height & volume
  • Protocol steps with CV
  • Chemical database (106)
  • Auto MW → weight
Model V = CV·Vcol  |  W = C·MW·V
PDF report
Open calculator

Buffer Recipe

Hit a target pH every time — weigh, titrate, or mix stocks, with temperature and ionic-strength handling.

  • 27 preset systems + custom
  • pH, concentration & volume
  • 4 preparation methods
  • Temperature & ionic strength
Model pH = pKa + log([A−]/[HA])
PDF report
Open calculator

HETP & Asymmetry

USP <621> column qualification: plates, HETP, reduced height, asymmetry, and van Deemter fitting.

  • Theoretical plates (N)
  • Reduced plate height (h)
  • Asymmetry factor (As)
  • van Deemter curve fitting
Model N = 5.54 × (VR/Wh)2  |  h = HETP/dp
PDF report
Open calculator

Protein A Process Economics

Compare capture scenarios side-by-side — mass balance, resin cost, and $/g against the column database.

  • Resin cost / campaign
  • Protein cost ($/g)
  • Multi-scenario comparison
  • Column auto-selection
Model Cost/g = (Presin × Vbed × (n+1)) / mass
PDF report
Open calculator

Frequently asked

Is AmChromaLab really free?

Yes. Every calculator is free to use, with no sign-up and no install — everything runs in your browser.

Does my data leave my device?

No. All calculations run locally in your browser. The site collects anonymous, aggregate usage statistics (pages viewed and which fields are used — never the values you enter). See the Privacy Policy.

What are the formulas based on?

Core methods are cross-checked against published references — HETP and theoretical plates follow USP <621>, and the open-bed pressure–flow model follows Stickel & Fotopoulos (2001).

Can I use it offline?

Yes. Pages degrade gracefully without a network connection, and the calculators keep working fully offline.

Disclaimer & formula validation

Disclaimer. This calculator is designed as a supportive tool for downstream processing (DSP) and preparative chromatography scientists, not associated with any third-party entity. It is intended to assist with calculations and should not supersede or replace your facility's internal protocols, standard operating procedures (SOPs), or official guidelines.

While we have made every effort to verify the accuracy of the formulas and results, the potential for calculation errors exists. Please verify critical parameters and exercise professional judgment when interpreting the outputs.

Formula validation. Core calculations are cross-checked against published methods and an internal test suite — HETP / theoretical-plate methods follow USP <621>, and the open-bed pressure–flow model follows Stickel & Fotopoulos (2001).

We are committed to continuously improving this tool. If you notice any discrepancies, have suggestions, or would like to share feedback, please contact the development team.

Privacy. All calculations run entirely in your browser. The site collects anonymous, aggregate usage statistics (pages viewed and which fields are used — never the values you enter) to guide improvements. Details: Privacy Policy.